A novel autophagy enhancer as a therapeutic agent against metabolic syndrome and diabetes.
Abstract
Autophagy is a critical regulator of cellular homeostasis, dysregulation of which is associated with diverse diseases. Here we show therapeutic effects of a novel autophagy enhancer identified by high-throughput screening of a chemical library against metabolic syndrome. An autophagy enhancer increases LC3-I to LC3-II conversion without mTOR inhibition. MSL, an autophagy enhancer, activates calcineurin, and induces dephosphorylation/nuclear translocation of transcription factor EB (TFEB), a master regulator of lysosomal biogenesis and autophagy gene expression. MSL accelerates intracellular lipid clearance, which is reversed by lalistat 2 or Tfeb knockout. Its administration improves the metabolic profile of ob/ob mice and ameliorates inflammasome activation. A chemically modified MSL with increased microsomal stability improves the glucose profile not only of ob/ob mice but also of mice with diet-induced obesity. Our data indicate that our novel autophagy enhancer could be a new drug candidate for diabetes or metabolic syndrome with lipid overload.
The Botanical Garden of Department of Pharmacognosy, University of Vienna, Austria. Photo by Atanas G. Atanasov.
Lagoon of the seven cities, a twin lake in the crater of a dormant volcano in the western part of the São Miguel island (Azores, Portugal). Photo by Dr. Ana Sanches Silva.
Central Australian desert scene. Photo by Dr. Rohan Davis.
HEK293T cells. Photo by Atanas G. Atanasov.
Tropical rainforest, Queensland, Australia. Photo by Dr. Rohan Davis.
Australian ocean scene. Photo by Dr. Rohan Davis.
Laboratory equipment. Photo by Atanas G. Atanasov.
Rhododendron arboreum flower. Photo by Devesh Tewari.
The Valley of Flowers, Himalaya, India. Photo by Dr. Rajeshwari Singh.
Bryce Canyon, Utah, USA. Photo by Dr. Anupam Bishayee.
Euphorbia prolifera Buch. (Ham) Ex. Photo by Devesh Tewari.
Foothills of Himalaya, India. Photo by Devesh Tewari.
The Valley of Flowers, Himalaya, India. Photo by Dr. Rajeshwari Singh.
Eco-School Festival, Leiria-Portugal, Spring 2018
Polygonum maritimum (L.) (Polygonaceae)- Ourdania beach (Ain Temouchent- west Algeria)- taken by PhD. EL HACI Imad Abdelhamid
Flower blossoms at gardens by the bay Singapore-photo by Dr. Hemanth Kumar Boyina.