Electrochemical Proteus vulgaris whole cell urea sensor in synthetic urine


An electrochemical whole cell Lab-on-a-Chip sensor for the detection of urea in synthetic urine has been developed. Urea was indirectly monitored by the electrochemical ammonia oxidation liberated by the microbial P. vulgaris microorganism surface membrane reaction. For this, a custom-made platinum miniature electrode platform utilizing lift-off photolithography technique and coupled with a 3D printed casing was fabricated. A P. vulgaris pellet was drop casted on the Pt working electrode surface and exposed to different urea concentrations in synthetic urine. The electrochemical determination of urea was done via the ammonia oxidation reaction. In presence of P. vulgaris, our results showed a relationship between the ammonia oxidation peak current density (from 0.5 to 5.0 μA/cm2) and the urea concentration (from 0.01 M to 0.1 M) in synthetic urine aqueous solutions. The sample volumes analyzed were 80 μL and 100 μL of urea in synthetic urine at pH 8.0. The former presented less variability and higher sensitivity. The as-prepared microchips were characterized by scanning electron microscopy, energy dispersive spectroscopy, X-ray photoelectron spectroscopy and cyclic voltammetry to determine its surface composition, modification, and robustness.

Read full text


Myreisa Morales-Cruz, Nadja E. Solis-Marcano, Claudia Binder, Craig Priest, Carlos R. Cabrera, Electrochemical Proteus vulgaris whole cell urea sensor in synthetic urine, Current Research in Biotechnology, Volume 1, 2019, Pages 22-27, https://doi.org/10.1016/j.crbiot.2019.07.002

Keywords: electrochemical Proteus vulgaris whole cell urea sensor, biosensors, synthetic urine, Lab-on-a-Chip sensor, #biosensor, #biotech.

The International Natural Product Sciences Taskforce (INPST) maintains up-to-date lists with conferencesgrants and funding opportunitiesjobs and open positions, and journal special issues with relevance for the area of phytochemistry and food chemistry, pharmacology, biotechnology, medicine and pharmacognosy research, and natural product science.

Join for free INPST as a member

INPST social media channels